human mammary epithelial cell line mcf10a  (ATCC)

Journal: Frontiers in Pharmacology

Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism

doi: 10.3389/fphar.2026.1706420

Figure Lengend Snippet: PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human normal mammary epithelial cells MCF10A (ATCC, USA) were cultured in DMEM/F12 (Procell) supplemented with HS (5%), EGF (20 ng/mL), hydrocortisone (0.5 μg/mL), insulin (0.5 μg/mL), NEAA (1%), and P/S (1%).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay

Journal: Hereditas

Article Title: PTTG1 regulates the TGF-β/Smad pathway to promote the growth and metastasis of gastric cancer cells

doi: 10.1186/s41065-026-00647-0

Figure Lengend Snippet: PTTG1 is highly expressed in gastric cancer cells. A Analysis of the TCGA database showing PTTG1 mRNA expression in gastric cancer tissues ( n = 408) and normal gastric tissues ( n = 211). B Western blot detection of PTTG1 protein expression in the normal gastric epithelial cell line GES-1 and gastric cancer cell lines SGC-7901, HGC-27, BGC-823, and AGS, with GAPDH used as a loading control. Quantification of PTTG1 expression relative to GAPDH is shown in the accompanying bar graph. Each experiment was repeated three times. Data are presented as mean ± SD. *** P < 0.001, ** P < 0.01 versus GES-1

Article Snippet: Human gastric cancer cell lines, including AGS and MKN-45, and normal gastric epithelial cells (GES-1), were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Hereditas

Article Title: PTTG1 regulates the TGF-β/Smad pathway to promote the growth and metastasis of gastric cancer cells

doi: 10.1186/s41065-026-00647-0

Figure Lengend Snippet: Silencing PTTG1 suppresses epithelial-mesenchymal transition in gastric cancer cells. Western blot analysis of EMT-associated markers (E-cadherin, N-cadherin, α-SMA, and Vimentin) in SGC-7901 and HGC-27 cells transfected with control, si-NC (scramble), or si-PTTG1. GAPDH served as the internal loading control. Densitometric quantification of protein expression relative to GAPDH is shown in bar graphs. Each experiment was repeated three times. Data are presented as mean ± SD. *** P < 0.001, ** P < 0.01 versus si-NC

Article Snippet: Human gastric cancer cell lines, including AGS and MKN-45, and normal gastric epithelial cells (GES-1), were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Transfection, Control, Expressing