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mcf10a human mammary epithelial cells  (ATCC)


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    ATCC mcf10a human mammary epithelial cells
    (A) 3D morphogenesis assays of <t>MCF10A</t> cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).
    Mcf10a Human Mammary Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates"

    Article Title: Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates

    Journal: bioRxiv

    doi: 10.64898/2026.01.29.702607

    (A) 3D morphogenesis assays of MCF10A cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).
    Figure Legend Snippet: (A) 3D morphogenesis assays of MCF10A cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).

    Techniques Used: Expressing, Infection, Construct, Cell Culture, Staining, Marker, Incubation



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    (A) 3D morphogenesis assays of <t>MCF10A</t> cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).
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    Image Search Results


    (A) 3D morphogenesis assays of MCF10A cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).

    Journal: bioRxiv

    Article Title: Analysis of the assembly, stabilization and maturation of the multiphasic TAZ biomolecular condensates

    doi: 10.64898/2026.01.29.702607

    Figure Lengend Snippet: (A) 3D morphogenesis assays of MCF10A cells expressing Flag-TAZ variants. MCF10A TAZ KO cells were infected with the various Flag-tagged TAZ (F-TAZ) constructs as indicated. These cells, as well as the parental MCF10A cells, were cultured in 3D Matrigel for 7 days and stained for α-integrin (green) as a basolateral marker and GM130 (red) as an apical marker. Nuclei were counterstained with DAPI (blue). Representative confocal images were from three independent experiments. Bar , 40 μm. (B) Quantification of average acinar sizes based on the number of nuclei per acinus. A total of 20–25 acini from three independent experiments were analyzed per cell line. Asterisks depict significant differences between the pairs indicated by the brackets (*, P <0.05; ****, P <10 −4 ; one-way ANOVA and Tukey’s post-hoc test). (C) Anchorage-independent growth in soft agar. MDA-MB-231 TAZ KO cells were infected with the designated F-TAZ constructs as indicated. Following infection, 5,000 cells were plated in 6-well soft agar plates and incubated for 21 days. Parental MDA-MB-231 cells and TAZ KO cells were used as controls. Colonies were stained with 1 mg/ml MTT. Bar , 100 μm. Colony numbers from each cell line expressing different TAZ constructs were counted and are shown in (D). Data are mean ± SEM of three independent experiments. Asterisks represent significant differences between the indicated pairs (****, P <10 −4 ; one-way ANOVA and Dunnett’s post-hoc test).

    Article Snippet: HeLa cells (cat. #CCL-2), MCF10A human mammary epithelial cells (cat. #CRL-10317), MDA-MB-231 human breast cancer epithelial cells (cat. #HTB-26) and 293T human kidney epithelial cells (cat. #CRL-3216) were from American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Expressing, Infection, Construct, Cell Culture, Staining, Marker, Incubation